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cre dependent aav backbone  (Addgene inc)


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    Structured Review

    Addgene inc cre dependent aav backbone
    Cre Dependent Aav Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 736 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cre+dependent+aav+backbone/pAAV-hSyn-DIO-hM3D(Gq)-mCherry+(Plasmid+%2344361)/bio_rxiv__64898__2026__03__26__714551-145-22-26
    Average 96 stars, based on 736 article reviews
    cre dependent aav backbone - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Amplification:

    Article Title: Molecularly defined subpopulations of leptin receptor neurons dissociate the control of food intake from blood pressure
    Article Snippet: To generate RADRR plasmids, rabies glycoprotein and TVAmcherry were individually PCR amplified using TRE-DIO-TVAmCherry-rG (Addgene plasmid # 166602) as a template. .. The resulting rabies glycoprotein amplicon was inserted into an Flp-dependent AAV plasmid backbone (AAV-hSYN1-fDIO-GFP) and the TVAmcherry amplicon was inserted into a Cre-dependent AAV backbone (AAV-hSYN1-DIO-hM3DqmCherry; Addgene plasmid #44361) using standard restriction enzyme and ligation approaches. ..

    Bioprocessing:

    Article Title: Molecularly defined subpopulations of leptin receptor neurons dissociate the control of food intake from blood pressure
    Article Snippet: To generate RADRR plasmids, rabies glycoprotein and TVAmcherry were individually PCR amplified using TRE-DIO-TVAmCherry-rG (Addgene plasmid # 166602) as a template. .. The resulting rabies glycoprotein amplicon was inserted into an Flp-dependent AAV plasmid backbone (AAV-hSYN1-fDIO-GFP) and the TVAmcherry amplicon was inserted into a Cre-dependent AAV backbone (AAV-hSYN1-DIO-hM3DqmCherry; Addgene plasmid #44361) using standard restriction enzyme and ligation approaches. ..

    Plasmid Preparation:

    Article Title: Molecularly defined subpopulations of leptin receptor neurons dissociate the control of food intake from blood pressure
    Article Snippet: To generate RADRR plasmids, rabies glycoprotein and TVAmcherry were individually PCR amplified using TRE-DIO-TVAmCherry-rG (Addgene plasmid # 166602) as a template. .. The resulting rabies glycoprotein amplicon was inserted into an Flp-dependent AAV plasmid backbone (AAV-hSYN1-fDIO-GFP) and the TVAmcherry amplicon was inserted into a Cre-dependent AAV backbone (AAV-hSYN1-DIO-hM3DqmCherry; Addgene plasmid #44361) using standard restriction enzyme and ligation approaches. ..

    Ligation:

    Article Title: Molecularly defined subpopulations of leptin receptor neurons dissociate the control of food intake from blood pressure
    Article Snippet: To generate RADRR plasmids, rabies glycoprotein and TVAmcherry were individually PCR amplified using TRE-DIO-TVAmCherry-rG (Addgene plasmid # 166602) as a template. .. The resulting rabies glycoprotein amplicon was inserted into an Flp-dependent AAV plasmid backbone (AAV-hSYN1-fDIO-GFP) and the TVAmcherry amplicon was inserted into a Cre-dependent AAV backbone (AAV-hSYN1-DIO-hM3DqmCherry; Addgene plasmid #44361) using standard restriction enzyme and ligation approaches. ..



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